The Cubic Lipid Phase and Bacteriorhodopsin / Single Project

Media Details

Media ID: SYM0033
Client: Technical
Created on: 2004-02-22
Published on: 2004-03-11
Dimensions: 1500 x 1500 pixels

Type: Illustration

Audiences: Technical, Scientific

Keywords: bacteriorhodopsin, br, clp, lcp, cubic, lipid, phase, crystallization, transmembrane, protein, ion, pump, bicontinuous, water, lateral diffusion

Order or Customize it
  • The Cubic Lipid Phase and Bacteriorhodopsin

Description

The cubic lipid phase (CLP, aka LCP) is a novel type of method for membrane protein crystallization. The CLP is composed of a bicontinuous surface (bilayer lipids, typically monoolein), of an average surface curvature of zero (minimal surface), that creates two mutually exclusive solvent compartments.

A detergent-solubilized protein such as bacteriorhodopsin (BR, shown in magenta surrounding by 'green' detergent) is exposed to the CLP environment and integrates itself into the bilayer, most likely due to an energetic gain between membrane protein and lipid, versus membrane protein and detergent.

The CLP-incorporated protein is believed to act as a reservoir for growing crystals.

References

None provided